Lazio was hit with a $122,00 punishment after sections of its support sang "Juden Tottenham" ("Tottenham Jews" in German) at the visiting fans, whose club has a historical Jewish connection.
They also held up a banner which read "Free Palestine" during the game on November 22 last year.
Tottenham was also fined $13,500 for crowd disturbances in the same fixture.
Lazio had previously been fined $54,500 by UEFA after monkey chants were directed at Tottenham players during the reverse fixture in London in September.
UEFA also punished Lazio after their fans threw missiles and fireworks on to the pitch while the team was sanctioned for their late arrival at the stadium.
The Italians also received a suspended one-match stadium ban and a further $68,000 fine for their supporters' racist conduct in the Europa League group stage game against Maribor in Slovenia on December 6.
Maribor were fined $23,000 for poor match organization and the throwing of missiles and fireworks by their supporters at the same Group J game.